Sybr Green for qRT-PCR - Use of Sybr Green in optical cyclers (Mar/24/2004 )

I want to prepare a pcr mixture with sybr green and use it in an optical thermal cycler. Does anyone know where to purchase the dye that comes in a compatible solution for PCR? and cheap. Thanks.

Why don't you just use some ready-to-use kit such as kit from Applied biosystems or qiagen

http://www.appliedbiosystems.com/catalog/m...112235&trail=no



If cost is a problem and you want to prepare your mixture, there is a company in china selling PCR grade SYBR green I (cat #SS-7582) at the price about $80 for 5X500ul (~200 pcr reactions). you can find the info here
http://www.ope-tech.com/products/SYBR_Green2.htm