MFI ratio - (Aug/03/2007 )
Hi everybody!
I'm having problem to analyse my FACS data. I would like to ask what is the best way to assure the increase of expression.
Some people use the MFI ratio (substracting background fluorescence of negative/isotype control from the MFI of the sample), and others use MFI sample from positive gate/MFI sample from negative gate.
I've heard about MFI positive / (MFI positive - MFI negative). I'm really confused 
. Which should I use?? Which is the most informative value??
Thanks in advance
Dear Beata,
I don't think there's a right and wrong way here, it really depends what you are trying to show. But I personally would always prefer to see the actual FACS histograms of both populations overlaid, to let me judge myself if I think there's a difference.
Best wishes
LeserattePD
I'm having problem to analyse my FACS data. I would like to ask what is the best way to assure the increase of expression.
Some people use the MFI ratio (substracting background fluorescence of negative/isotype control from the MFI of the sample), and others use MFI sample from positive gate/MFI sample from negative gate.
I've heard about MFI positive / (MFI positive - MFI negative). I'm really confused
. Which should I use?? Which is the most informative value??Thanks in advance
PS I hope you don't mean that you are trying to compare values from samples done in different experiments... because that would be a very big NoNo, unless you do very strict calibrations.
I don't think there's a right and wrong way here, it really depends what you are trying to show. But I personally would always prefer to see the actual FACS histograms of both populations overlaid, to let me judge myself if I think there's a difference.
Best wishes
LeserattePD
I'm having problem to analyse my FACS data. I would like to ask what is the best way to assure the increase of expression.
Some people use the MFI ratio (substracting background fluorescence of negative/isotype control from the MFI of the sample), and others use MFI sample from positive gate/MFI sample from negative gate.
I've heard about MFI positive / (MFI positive - MFI negative). I'm really confused
. Which should I use?? Which is the most informative value??Thanks in advance