in vitro apoptosis and secondary necrosis - (Jun/29/2007 )
Hi everyone,
I'm a bit confused with the mechanism of apoptosis in vitro. According the many resources, cells that undergo apoptosis will go through secondary necrosis after extended incubation time. But If the cells undergo apoptosis, they will be phagocytosed by the neighbouring cells. If that's the case, how would secondary necrosis happen?
Please help.
Thank you!
I'm a bit confused with the mechanism of apoptosis in vitro. According the many resources, cells that undergo apoptosis will go through secondary necrosis after extended incubation time. But If the cells undergo apoptosis, they will be phagocytosed by the neighbouring cells. If that's the case, how would secondary necrosis happen?
Please help.
Thank you!
a necrosis of apoptotic cells is optional, phagocytosis of apoptotic bodies is the very final of apoptosis step w/o necrosis
hi!! i am not sure what is secondary necrosis,but i think it this way : normal cells require apoptosis for proper programmed cell death and cell differetiation, excessive apoptosis will cause traumatic cell death or living tissues [this is necrosis] , and usually cell death by necrosis do not transmit signals to macrophages [cells that engulf dead cells` debris] , as a result of chemical agents released by these debris, necrosis continues .
what do you mean by "normal cells require apoptosis for... cell differentiation"?
(surely some tissues or organs but single cells?)
most apoptotic bodies are absorbed by their own tissue not macrophages as implied in your answer
oh... I made mistakes...I think what I was saying was normal cells are programmed to cell death by nature, but this programmed cell death cannot be too much.and the part about macrophage was wrong, I should say that if cell death was caused by infections not by natural programmed cell death, then the cell debris would be engulfed by macrophage... hope I got it right this time.... 