TO determine signalign pathway. - (Jun/15/2007 )
TO determine cell signaling pathway about interested protein,
we are going to treat siRNA or inhibitor of that. which do you think better method siRNA or inhibitor??
-shi21ne-
It's hard to say without knowing what protein you are trying to inhibit. If there is a commercially available inhibitor specific for your protein only, then I'd say that would be easiest. siRNA is always tougher in my opinion, especially if you haven't done it before.
Amanda
-Cheamps-
siRNA at best would knockdown 90-95%, so there is still some doubts. But if there is a specific inhibitor, then that might be better. make sure the inhibitor doesnot activate or inhibit some other protein, which is possible as with many of the pharmacological drugs.
-scolix-