charcol stripped FCS? - (Jan/07/2004 )
what is left in the serum after the charcol stripped treatment?
Charcoal stripped serum is treated by filtering chilled serum through an activated carbon adsorbent filter to remove non-polar material. This treatment removes lipophilic material but has little effect on the concentration of salts dissolved in the serum.
Charcoal stripped serum is steroid free and used for steroids and steroid receptor research.
anyone can tell me a really efficient protocol to remove estrogen from FCS with charcoal dextrane? I tried with two form literature but could remove only 10% estadiol