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annealing oligos for EMSA - (May/18/2007 )

A really really simple question here - I am annealing oligos (27bp each) for EMSA. If I anneal, say 100 pmoles each oligo, how many pmoles do I have of double-stranded DNA?

I want to compare two different probes and calculate the specific activity for each, so I am assuming I need the same pmoles of ds DNA.




100 pmoles.