Calculation for xenograft tumor weight on nude mice - (May/09/2007 )

Dear all,

I would like to know if anyone has heard of an equation for calculation of s.c. xenograft tumor on nude mice as:

tumor weight = pi/6*L*W*W where L = length W=width

Did anyone calculate tumor weight at all...

Many thanks,
Kallichroma

The most important thing is the difference between experimental and control group in tumor growth no matter what method is used to estimate how fast tumors grow. Either volume or weight is derived from the same mesurements of length and width, and they won't affect the comparison. The most accurate mesurement is the weight of removed tumors at the end of an experiment.

Hi!

I agree with Pcrman, there are many different methods to estimate tumor volume in dynamic . You could simple measure like this - V=width*length*depth, but you should use this formula in all of your calculations concerning experimental and control groups. And IMPORTANT NOTE only one person should measure this parameters (width*length*depth) during this animal experiment. Never let another person to make this measurements.

I wouldn't assume you can use that equation to get an accurate weight, unless it was designed specifically for your tumor type

even with the same tissue-type, injection amt, etc, the tumors in a given batch of mice will not have the same density. I think this would affect thier weight, and you can't base it just on volume

Here is a paper describing different formulas to estimate tumor volume.
raj

Thank you, rajgene

This article is very useful.

[quote name='rajgene' date='Jul 10 2007, 03:49 AM' post='104213']
Here is a paper describing different formulas to estimate tumor volume.
raj

Thank you Rajgene!

You all are always welcome.