not equilibrium HW microsatelites - (Apr/02/2007 )
Hi:
I am using microsatellites marker for parentage analysis, some of them are not in HW equilibrium, this can be a problem in my analysis or in the future analysis???(eg. pedigree reconstruction)
Regards
Ro
-ro242-
Hi ro242,
Yes. Many things can lead to HW disequilibrium, but with microsatellites, I would first suspect that you have null alleles - alleles that are failing to amplify in your PCR. If null alleles are common in the population, the failure to detect them can lead to false (or at least inaccurate) conclusions. Try different primers and see what you get.
-wbla3335-
QUOTE (wbla3335 @ Apr 3 2007, 12:27 AM)
Hi ro242,
Yes. Many things can lead to HW disequilibrium, but with microsatellites, I would first suspect that you have null alleles - alleles that are failing to amplify in your PCR. If null alleles are common in the population, the failure to detect them can lead to false (or at least inaccurate) conclusions. Try different primers and see what you get.
Yes. Many things can lead to HW disequilibrium, but with microsatellites, I would first suspect that you have null alleles - alleles that are failing to amplify in your PCR. If null alleles are common in the population, the failure to detect them can lead to false (or at least inaccurate) conclusions. Try different primers and see what you get.
Thanks for you reply; i will get in consideration and a will try with other primers.
Regards
Ro
-ro242-