MatriGel plating - (Mar/21/2007 )
Anyone know of any substitutes to matrigel?
I've recently come across a company called Glycosan Biosystems (www.glycosan.com). They've got a semi-synthetic hydrogel that works nicely. You don't have to work with the gel on ice, and there are no pH adjustments.
Hello all,
I am lost. I don't understand why we should coat the plates with matrigel and what type of cells need that? I have grown some cells for animal studies. The day of injection, I trypsinized the cells and mixed cell suspension + Matrigel for injection. I handled Matrigel exactly the same way in this discussion - on ice, e.t.c. and that project was successful. But I don't understand this discussion. Just interested.
Thanks.
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Padma, we coat plates with matrigel to perform in vitro experiments.
Matrigel can be both used in vivo and in vitro.
hi
I haven´t used it, but Millipore also sells Matrigel, they call it ECL Cell Attachment Matrix (ref. nº 08-110)
There is an alternative to BD's Matrigel. It is from a company called Trevigen. They sell basement membrane extract in a line called Cultrex. It works the same and I have found it to be more reproducable in my cell cultures.
Anyone know of any substitutes to matrigel?
I've recently come across a company called Glycosan Biosystems (www.glycosan.com). They've got a semi-synthetic hydrogel that works nicely. You don't have to work with the gel on ice, and there are no pH adjustments.
Jayster, I am going to try with with this gel from Glycosan. Have you had already an experience with it?Does it work well? Is this solution cheaper than Matrigel?