New to Area. - (Mar/19/2007 )
Hi,
I'm new to the area and was wondering about SSI treated DNA. From what I can gather, it can be used as a positive control for methylation status as it methylates DNA?? Is that right??
I need to use it as a control in a PCR for Bisulfite treated DNA.
Any help would be greatly appreciated.
C
-Casey-
Hi Casey,
welcome to a new and exciting field.
SssI methylase DNA can be used as a control, but it has to also be bisulfite treated as well as your samples.
Good lcuk
Nick
-methylnick-