Post-chromosomal supernatant - what is it?! - (Mar/19/2007 )
I've found this expression in several papers but didn't find an exact definition. Can anyone help me what does post-chromosomal supernatant means? How it has to be prepared? What kind of cell components it contains?
Thanks a lot!
Thanks a lot!
I think it is the same as the also often used "postnuclear" supernatant which means the supernatant after only pelleting the nuclei (which bear the chromosomes) during subcellular fractionation
uhh, so only the chromosomes are missing from the cell lysate?
normally you do it by differential centrifugation; may be some larger debris are co-pelletd with nuclei; but during preparation nuclei should be intact; if they are lysed or homogeneized, simple non-gradient centrigugation to fractionize becomes difficult...
sorry, I'm really not in this fiedl but I want to know: what components can be found in a postnuclear supernatant, and what goes to the pellet?
you can pellet in density gradient and non-gradient centrifugation; postnuclear supernatant can be achieved by at least 1000xg for 10 min in a non-gradient centrifugation; in the postnuclear supernatant remain mitochondria, lysosomes, microbodies, various membrane vesicles and cytoplasm; the higher the g-force the less chromosomes and other organelles you have
Now I think it is clear. Thanks!