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showing CPE by Giemsa staining - (Mar/12/2007 )

Hi;

İs anyone knows Giemsa staining method for showing CPE in cell culture flask?

Best regars.

-degirmenci-

may this help you!!!!!!!!!!!!!








Giemsa stain


i) Preparations of tissue culture supernatant, smears or impressions of the kidney, liver, and spleen should be prepared, air-dried, and fixed for 5 minutes in absolute methanol.


ii) Immerse slides in a working solution of Giemsa stain for 30 minutes.


Stock solution: 0.4 (w/v) in buffered methanol solution, pH 6.9 (commercially available).


Working solution: diluted 1/10 in phosphate buffer pH 6.0 (0.074 M NaH2PO4, 0.009 M Na2HPO4).


iii) Destain with tap water.


iv) Observe slides under oil immersion. Tissue smears from infected organs show darkly stained pleiomorphic organisms occurring in coccoid or ring forms, frequently in pairs, with a diameter of 0.5-1.5 ┬Ám.









QUOTE (degirmenci @ Mar 12 2007, 04:04 PM)
Hi;

İs anyone knows Giemsa staining method for showing CPE in cell culture flask?

Best regars.

-Amit nayak-