general flow cytometry protocol - (Mar/05/2007 )

hi,

can anybody know general flow cytometry protocol staining cells with molecular probes. for example i am i have to incubate my cells with probe and then i have to analyse cells. but how much probe and incubation time i dont know. please tell if anybody know about this

Hi where are you from

Amount of probe depends on your experiment. We always determine how much of which antibody we have to use when a new stock arrives.
Apart from this, is it intracellular or extracellular, do you have to fix cells or not, what colors will you be using?