# Unit conversion mol -> gram - (Mar/01/2007 )

If I have a enzyme 50kDa and I know that the concentration of said enzyme is 140pmol/mg in a total protein conc of 12.785 mg/ml how do I convert the amount of enzyme to grams? Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

I am utterly confused when it comes to unit conversion!!!

-grahamkeith-

you may wish to try this tool

for the first part, though, it's quite difficult if you don't have a total volume

-aimikins-

QUOTE (grahamkeith @ Mar 1 2007, 10:45 AM)
If I have a enzyme 50kDa and I know that the concentration of said enzyme is 140pmol/mg in a total protein conc of 12.785 mg/ml how do I convert the amount of enzyme to grams? Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

I am utterly confused when it comes to unit conversion!!!

140*12,785=1789,9pmol/ml
1789,9*50000= 89,495mkg/mll

Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

so much that in 100mkl but concentration decrease 20 times. 89,495/20=4,47mkg/ml

-circlepoint-

QUOTE (circlepoint @ Mar 1 2007, 12:19 PM)
QUOTE (grahamkeith @ Mar 1 2007, 10:45 AM)
If I have a enzyme 50kDa and I know that the concentration of said enzyme is 140pmol/mg in a total protein conc of 12.785 mg/ml how do I convert the amount of enzyme to grams? Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

I am utterly confused when it comes to unit conversion!!!

140*12,785=1789,9pmol/ml
1789,9*50000= 89,495mkg/mll

Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

so much that in 100mkl but concentration decrease 20 times. 89,495/20=4,47mkg/ml

what is this mkg and mkl business? I have never seen this SI?

Thankyou for your effort it seems clearer now.

-grahamkeith-

QUOTE (aimikins @ Mar 1 2007, 12:15 PM)
you may wish to try this tool

for the first part, though, it's quite difficult if you don't have a total volume

What a great tool! thanks. The total volume in this example was 2ml

-grahamkeith-

yeah, but I didn't know if it also pertained to the first part of the question

I thought that you were wanting the total grams of protein in the sample (in which case you'd need the volume), but when I re-read your post I guess you were looking for the concentration in terms of grams

-aimikins-

QUOTE (grahamkeith @ Mar 1 2007, 12:39 PM)
QUOTE (circlepoint @ Mar 1 2007, 12:19 PM)
QUOTE (grahamkeith @ Mar 1 2007, 10:45 AM)
If I have a enzyme 50kDa and I know that the concentration of said enzyme is 140pmol/mg in a total protein conc of 12.785 mg/ml how do I convert the amount of enzyme to grams? Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

I am utterly confused when it comes to unit conversion!!!

140*12,785=1789,9pmol/ml
1789,9*50000= 89,495mkg/mll

Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter????

so much that in 100mkl but concentration decrease 20 times. 89,495/20=4,47mkg/ml

what is this mkg and mkl business? I have never seen this SI?

Thankyou for your effort it seems clearer now.

Excuse, mkg is ug and mkl is ul

-circlepoint-

QUOTE (aimikins @ Mar 1 2007, 01:16 PM)
yeah, but I didn't know if it also pertained to the first part of the question

I thought that you were wanting the total grams of protein in the sample (in which case you'd need the volume), but when I re-read your post I guess you were looking for the concentration in terms of grams

Hi aimikins!

what do you mean "concentration in terms of grams"?

-circlepoint-

concentration in terms of grams = grams/ml
concentration in terms of moles = moles/ml

do you see?

-aimikins-