PCRÇóÖú - (Jun/02/2003 )
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I assume that you are doing RT-PCR. Many thing could have gone wrong with your experiment. Here are some you want to check.
1) RNA quality. You can run a RNA gel to verify you RNA is good or not
2) Do a RT-PCR with your RT reaction using some primers you know they worked before, such as house-keeping gene (beta-actin, 18S rRNA, etc), if your RT is good, you should get something, if not, your RT is bad.
3) If you can get bands with control primers, then either there is no expression of your gene in the samples you studied, or something is wrong with your primers.
4) Test your new primers with known good RT.
5) Double check your primer sequence with genebank sequence, best using RefSeq.
Hope it helps.
Hula
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