how should I Reconstitute my Fibronectin product - (Feb/13/2007 )

hello everyone

I bought the Fibronectin from bovine plasma (F1141 Sigma) for use as a cell culture substratum. The product is already 1 mg/mL (in 0.5 M NaCl, 0.05 M Tris, pH 7.5); following the literature I should reconstitute it into my prefered concentration.

But I don't have enough knowledge about what kind of buffer solution I should use to dilute this product. Must I use buffer of 0.5 M NaCl, 0.05 M Tris, pH 7.5 or I could also use PBS, which is more easy to prepare. The way I use the fibronectin solution is putting 200ul fibronectin solution on my glass slips and incubate for 1h, then wash with PBS 3 times; then I will seed my cells on such substrate.

Thanks a lot; look forward to your help! I need it for tomorrow. URGENT§§

Hi, Your Fibronectin will be happy in PBS.

Do not freze thaw it, aliquot the stock and freeze appropriate volumes to dilute as needed.

Hope this helps

A

but another question is how to conserve it? Actually this FN product originally is in NaCl qnd tris buffer; in the bottle there is marked "stored in 4 to 8 degree". But I normally would like to store it as long as possible; because I will use it not so often. If I could store them in minus 20 degree, which will help store longer?? Especially after I reconstitute them in aliquot, how I should store them, in 4 degree or in minus 20 degree, to conserve it longer?

thanks