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how should I Reconstitute my Fibronectin product - (Feb/13/2007 )

hello everyone

I bought the Fibronectin from bovine plasma (F1141 Sigma) for use as a cell culture substratum. The product is already 1 mg/mL (in 0.5 M NaCl, 0.05 M Tris, pH 7.5); following the literature I should reconstitute it into my prefered concentration.

But I don't have enough knowledge about what kind of buffer solution I should use to dilute this product. Must I use buffer of 0.5 M NaCl, 0.05 M Tris, pH 7.5 or I could also use PBS, which is more easy to prepare. The way I use the fibronectin solution is putting 200ul fibronectin solution on my glass slips and incubate for 1h, then wash with PBS 3 times; then I will seed my cells on such substrate.

Thanks a lot; look forward to your help! I need it for tomorrow. URGENTĀ§Ā§

-lilylille-

Hi, Your Fibronectin will be happy in PBS.

Do not freze thaw it, aliquot the stock and freeze appropriate volumes to dilute as needed.

Hope this helps

A

-Techqueen-

QUOTE (Techqueen @ Feb 13 2007, 08:39 AM)
Hi, Your Fibronectin will be happy in PBS.

Do not freze thaw it, aliquot the stock and freeze appropriate volumes to dilute as needed.

Hope this helps

A



thanks a lot for quick answer. this help is really in time and in need.

-lilylille-

but another question is how to conserve it? Actually this FN product originally is in NaCl qnd tris buffer; in the bottle there is marked "stored in 4 to 8 degree". But I normally would like to store it as long as possible; because I will use it not so often. If I could store them in minus 20 degree, which will help store longer?? Especially after I reconstitute them in aliquot, how I should store them, in 4 degree or in minus 20 degree, to conserve it longer?

thanks

-lilylille-