Protocol Online logo
Top : Forum Archives: : Cell Biology

Biochem. protocol - albumin deplition (Feb/09/2007 )

can anybody provide me mannual protocol for albumin deplition from the serum sample ? please help me out .

-Amit nayak-

QUOTE (Amit nayak @ Feb 10 2007, 07:33 AM)
can anybody provide me mannual protocol for albumin deplition from the serum sample ? please help me out .


I haven´t used them but there are some HPLC columns that can deplete albumin from your samples and I think that there are also commercially available some kits to deplete it (if I´m not wrong, Beckman Coulter has some of them, but no cheap).
But in my opinion, the cheapest procedure is to inmunoprecipitate albumin in the same way as another protein, with the difference that now what you discard is the inmunoprecipitate containing albumin.

-Pumuki-