Help! GFP transfection - (Jan/25/2007 )
QUOTE (scolix @ Jan 28 2007, 06:13 PM)
QUOTE (Kathy @ Jan 28 2007, 06:26 PM)
we use new born or postnatal mice here to prepare SCG neurons for transfection. embryonic mice will mean we have to kill the mother and this is not an option here 
However, I do think that PEI is not very effecient for SCG.
i cant get repeatable results and dont like it. but to transfect with virus i need to clone my gene again into the virus genome right? 
However, I do think that PEI is not very effecient for SCG.
i cant get repeatable results and dont like it. but to transfect with virus i need to clone my gene again into the virus genome right? Kathy,
You will need to clone your gene into a viral vector and you will have to make the virus, it might not be the best option for you if you wish to complete your project within a short time (within a month).
i see. thank you Scolix!
-Kathy-
Kathy ,
I read a paper where they have transfected hippocampal neurons 7 DIV using Qiagen's Effectene. May b its helpful.
-scolix-
QUOTE (scolix @ Jan 30 2007, 11:48 AM)
Kathy ,
I read a paper where they have transfected hippocampal neurons 7 DIV using Qiagen's Effectene. May b its helpful.
I read a paper where they have transfected hippocampal neurons 7 DIV using Qiagen's Effectene. May b its helpful.
i think that ordering something new is not an option here either...
thank you anyway! -Kathy-