Apoptosis assay - other than FACS (Jan/12/2007 )
I'm planning to run some experiments and I need to do an apoptose assay. I'm sure that some of the cells will go into apoptosis, but there must be some living cells left.
I also need to have a sort of quantification in this assay, so the percentage of cells into apoptose.
The problem is that I can't make use of a FACS. I can label the cells with annexinV and PI and count them under a fluorescent microscope, but I just have too many samples in this experiment.
Is it possible to quantify with DNA fragmentation?
And I also saw some kits, but I could not find any with a baseline and I need a percentage.
Do you have methods/alternatives/tips/tricks/kits?
I love the Roche Cell Death Elisa which quantifies the fragmented DNA released into cytoplasmic fraction. Easy and reliable
this Roche ELISA kit detects mono- and oligonucleomsomes (DNA +histone); the Roche Cell cytotoxicity assay detects LDH released from necrotic cells; necrosis should be measured in parallel; also do viability tests such as MTT/MTS and SRB assays