isolation of streptococcus faecalis - (Dec/15/2006 )

hello
i am trying to isolate s.faecalis from yoghurt.none of the media that i have tried till now have worked......the main problem being overgrowth by lactobacillus.have tried aerobic conditions,high ph(9.6)...but have not been successful.
can anyone suggest any medium as well as growth conditions?

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Strep. faecalis is now know as Enterococcus faecalis.

As fas as I remember there selective media for E. faecalis when it's isolated form the gut.

The media descripbed in the following pages might point you in the right direction
http://service.merck.de/microbiology/tedis...05262_0500.html
http://www.pubmedcentral.nih.gov/articlere...ubmedid=4214072

It would also be good to tell us which type of media you used.

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thanks for the links.it will be of much help.
the medium i used is my own modification of chesbro and evans (1959).......proteose peptone 1%,yeast extract0.5%,glucose 10%(autoclaved separately),dipotassium hydrogen phosphate 0.1%,NaCl 0.25%,azide 0.025%,agar 1.5% pH 9.6.
the azide concentration and the alkaline pH wasnt successful in preventing lactobacillus overgrowth.today i have inoculated from serially diluted yoghurt into lactic bacteria differential agar(mc donald et al AEM 1987,53:1382)) with some modifications .heterofermentative enterococcus faecalis should grow as bluish green colonies in the next 24 hrs......am i on the right track or is this gonna be my roadblock

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