Dissolving Type I collagen - (Dec/12/2006 )

Hi. I have bovine achilles tendon collagen I which i have added to 50mMTris-HCl (ph7.5), with 5mM CaCl2 , to a final concentration of 1mg/ml. It doesn't dissolve. Does any one know how to dissolve type I collagen?

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i have wokred with calf tendon type I collagen before,

you will have to dissolve it in 0.2M acetic acid, on a stirrer at 4 degrees, time taken to dissolve will depend on amount of collagen you have.

then prior to use you need to neutralise it with sodium hydroxide to an acceptible pH

hope this helps

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I followed the same kind of protocol for collagen I extraction from rat tails tendons. After stirring at 4°C, I centrifuged my solution at 14000rpm 4°C for 1 hour to discard waste.

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