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thawing in -20C for overnight - is this harmful (Nov/30/2006 )

hi all, i just want to ask that i have kept the cells for thawing in -20C for overnight, whether this will harm the cells?

-hepg2-

I dont think that thawing will harm your cells or significantly reduce viability of your cells.

-ThapaDinesh-

QUOTE (hepg2 @ Nov 30 2006, 10:15 AM)
hi all, i just want to ask that i have kept the cells for thawing in -20C for overnight, whether this will harm the cells?


is it bacteria or eucaryotes? was freeze medium still frozen or liquid? for bacteria I donĀ“t mind, but eucaryotes may be critical, some are definitely sensitive

-The Bearer-

I agree with Kosmodrom, I would not do it for eucaryotes.
bacteria are much more resistant.

-Missele-

Always freeze cells as slowly as you can and do the opposite for thawing.

-genehunter-1-

i wonder why you thaw cells in -20C ,most of us do this at 37-40C.

-wing111-

Depending on what type of cells you work with. I know for embryonic stem cells, thawing is performed at 37C and as quickly as possible. Thawing slowly for prolonged time is to be avoided.

--Jessica

-Jules7-