thawing in -20C for overnight - is this harmful (Nov/30/2006 )
hi all, i just want to ask that i have kept the cells for thawing in -20C for overnight, whether this will harm the cells?
I dont think that thawing will harm your cells or significantly reduce viability of your cells.
is it bacteria or eucaryotes? was freeze medium still frozen or liquid? for bacteria I don´t mind, but eucaryotes may be critical, some are definitely sensitive
I agree with Kosmodrom, I would not do it for eucaryotes.
bacteria are much more resistant.
Always freeze cells as slowly as you can and do the opposite for thawing.
i wonder why you thaw cells in -20C ,most of us do this at 37-40C.
Depending on what type of cells you work with. I know for embryonic stem cells, thawing is performed at 37C and as quickly as possible. Thawing slowly for prolonged time is to be avoided.