Implant tumor in nude mice - (Sep/12/2006 )
Use Romos cell for example. My implantment successful rate is only 50%. I use 1-cc syringe and a 27 gauge needle and inject cells subcutaneously (s.c.) into the lower flank of the mice. What's wrong? Please help me. Thank you!
May be the Romos cell is not healthy...they should be in their growth phase.
may be the mice reject the cell...choose different cell lines or mice.
Hope this may help.
before implantment, how do you mix the cells?
You can have a look at this protocol on this site, or better read the reference cited in the protocol which has some very nice tips.
I found that resuspending cells in PBS yields better result than in culture medium. Handling cells very gently such as minimizing exposure to trypsin digestion, centrifuging at low speed, dispersing cells gently.
Cell injection technique is also critical. Cells should be injected subcutaneously, not intradermally. There is a paper telling why. When withdrawing cells into the syringe, don't have the needle on the syringe.
Hope that helps.