Fetal Bovine Serum - (Sep/09/2006 )

I heard that uninactivated fetal bovine serum can cause apoptosis and cell death? is it true?

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What cell type are your working with? For your other post, can you send your cell photo as an attachment to us, rather than we send you photos?

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Yes, it's true!
In uninactivated serum you find proteins of complement system (a part of the immune system) that can damage the cells and lead to cell death.
To inactivate serum (in fact, to inactivate complement system), just incube it at 56°C for 30min.

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What cell type are your working with? For your other post, can you send your cell photo as an attachment to us, rather than we send you photos?
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Rat cortical cells.

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Yes, it's true!
In uninactivated serum you find proteins of complement system (a part of the immune system) that can damage the cells and lead to cell death.
To inactivate serum (in fact, to inactivate complement system), just incube it at 56°C for 30min.
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Thanks. Beautiful.

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