superoxide measurement - lucigenin method question (May/25/2002 )
Hi all! I was wondering if anyone has had problems using diethyldithiocarbamate as a SOD inhibitor in the lucigenin assay for superoxide? DDC seems to only be effective in my system for about 2 hours then precipitates. Does anyone know of an alternative SOD inhibitor and/or superoxide detection method other than ESR?
U can go for Cytochrome C reduction method to measure Superoxide oins.
Want more i can provide u later