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help in transfection of NHFs - (Aug/23/2006 )

Im doing transfection with normal human fibroblasts.My problem is that cells transfected with my clone r not showing any flourescence.However the control is okay.Does it mean that my clone is not good?But i've done sequencing & it shows that the sequence of the clone is alright.Plzzz help me.


You are going to tell us more about your experiment first.