Yeast Contamination - Need to get rid of the yeast (May/03/2002 )

I've made a cell line in Helas that is Tet inducible.  As luck would have it, my flasks became contaminated with yeasts before i had a chance to freeze it down.  I don't know where the yeast came from, but it was the only flask contaminated.  I've already taken the necessary decontamination steps of hood/incubator.  But is there a way to get rid of the yeast from my culture?

in the air, because yeast can sporulate and spores can stay in aerator filter. Look if you have filter near from culture cells.

This is a trouble-some case. What you can try (no guarantee for success):
1) wash cells 5 x with BSS containing anti-yeast drug (Amphotericin or Nystatin, available from SIGMA Aldrich)
2) Incubate cells with medium plus the drug for 48 hrs.
3) Remove drug to check whether treatment was successful.
Note: this will stress the cells a lot -risk of loosing the culture. Hence don't try it on your only culture.