centrifuge of cells - how to (Feb/23/2002 )
How long and how fast should a solution be centrifuged to obtain a pellet of epithilial cells.
The standard method I use (for connective tissue cells, primary and transformed) is 5 to 10 minutes at 180g.
I would expect this to work for epithelial cells too
i use 400g for 5 mins
400g for 7 minutes is the most optimum, I find