Need protocol for replication banding! - (Jul/31/2006 )

In my study , i will use the replication banding, but i did not use this method! So who can help me!
Thank you very much !

Do you want to do G-banding (early pulse BrdU) or R-banding (late pulse BrdU) and in what sort of tissue?

Thank you.
I want to do early replication pattern (early pulse Brdu),and late early replication pattern(late pulse Brdu).
cell line
do you have the protocol

Check your PM

I received ,thank you very much!