Does it need cotransfection vector when retrovirus transfected hek293? - (Jul/19/2006 )
,hi,everyone,I need help,I want to transfect HEK293 cell with retrovirus vector,Does it need an extra cotransfect vector,for example,env expressing vector?on the other word,does HEK293 contains env expressing gene?
oh,I meen I want to use HEK293 as packing cell to produce retrovirus.
The original 293 does not carry env. Are you working with packaging cell lines derived from 293?
for infection by a virus, no need to cotransfect.
If you want to transfect a plasmid and ask your cell to produce retrovirus, you need a packaging cell line. 293cells are not able to carry that. But phoenix cells = 293PACK are ok
I want to choose a suitable packaging cell line to package my retravirus vector inserted by goal gene,then I let the virus to infect a leukemia cell line,someone tell me HEK293a meet my demand,but,only HEK293,ad293,pa317 is easily available,I want to know which one I can use.
U need to co-transfect with a envelope protien, gag,pol,and rev plasmids along with ur vector containing the transgene.
There r different packaging cell types, we use 293T or 293FT.
Thanks a lot,Can i use pa317 to produce retrovirus dirrectly without cotransfection?Is it suitable to infect a human cell line with virus produced by mouse cell line?
U need to co-transfect with other plasmids unless the cells stably expresses these proteins.
I guess it shouldnt matter where the virus was produced. As we used produced virus in 293 and infected mice or rat.