cell harvest by scraping versus trypsinization - (Jul/13/2006 )
QUOTE (aimikins @ Jul 13 2006, 01:43 PM)
depends on what you're doing with the cells later, too
we work with HNEK. we use trypsin for most applications (passaging, internalization assays, etc), but we use scraping on ice for either protein or nuclear extraction protocols.
we work with HNEK. we use trypsin for most applications (passaging, internalization assays, etc), but we use scraping on ice for either protein or nuclear extraction protocols.
I am working with macrophage now. I want my cells to be intact after harvest. So which way is better, scraping or trypsinization? How long will it take to trypsinize macrophage? Thanks.
-yypang-