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start a methylation study - (Jul/06/2006 )

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PCR on bisulfite modified DNA is very tricky. First make sure your template DNA is good. You can try your DNA using some primers known to work. Hot-start your PCR is necessary and using some hotstart taq such JumpStart Red Taq from Sigma helps a lot.

As I said above, have you tried hotstarting your PCR? For me I cannot do without using JumpStart Taq from sigma.


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