Reconstituted Luciferin storage - (Jun/13/2006 )
Is luciferin stable if alliquoted and stored at -80 for the long term?
According to Promega:
(Cat.# E1483) or prepared by reconstituting Luciferase Assay Substrate with
Luciferase Assay Buffer. Luciferase Assay Reagent should be stored in aliquots
and is stable at –20°C for up to 1 month, or at –70°C for up to 1 year after reconstitution
or initial use. After preparation and freezing, the Luciferase Assay Reagent
should be mixed well before use. Nonreconstituted system components may be
stored at –20°C for 1 year. Store Luciferase Assay Substrate in the dark.
At a lab where I used to do my graduate thesis, they used to have dry stocks of luciferin, stored at -80°C, and they reconstituted it and aliquoted into single use entities, which they also stored @ -80, so I guess -80 would be the best to store it.