How Can I filtrate Curcumin solved DMSO?! - (Jun/07/2006 )
I have dissolved Curcumin in DMSO under non-sterile conditions (sterile conditions not possible when weighing the tiny amounts of curcumin). Now I want to use this in cell culture. But filtration of the liquid is not possible (due to the aggresive solvent of course). What can I do?
Do you think that some bacteria would survive in DMSO?
I know they shouldn't, but you never know ...just wanted to be on the safe side
Suggestions: 1) can you dilute it first, then filter it through with a .22 filter?
2) organic solvant resistant filter is available. check with VWR or Fischer Sci
I have experience with compounds solved in DMSO. I never filtered it (expecially for mixture due to loss of compounds in filter) and I had no contamination problem!
we routinely screen chemical compounds for anti bacterial and anti fungal activity. theses compounds are dissolved in DMSO and tested directly without filtration or sterilization.
you can directly use curcumin dissolved in DMSO.
all the best
I filter solutions dissolved in DMSO (my PI insists), using a 0.2um nylon membrane.
I never filtrated the component solubilized in DMSO because I don't know how much I would lose on the membrane. It's not only some few drops you lose in the filter, but molecules can bind on the membrane.
although, I never let the component more than 12 hours. I never saw any contamination.