new memeber:choose which ,hek293 or 293t? - (May/22/2006 )
hello everyone ,i am a new member of this forum from an old country
i am now begin to design my experiment ,i need to use 293 and transfection with psv-β-galactosidatse and recombinant of pgl3,and then harvest for luciferase assay system and β-glactosidase enzyme assay system .
although i have looked up some papers i donot know which kind of 293 cells i should choose for my transfection ,hek293 or 293t .exactly to say ,i donot know the difference between them and the research that they can be used .
thanks in advance !
293 cells is an immortal cell line.
The 293 T cell is the same cell lineage, in which the large T antigen from virus SV40 which stabilizes the p53 protein.
They're both ok for you, but i would choose first the Hek293 stain as it's less denatured than the 293T
Both of the 293 (HEK293 and 293T) should work perfectly fine for transfection. Stick to one of them for all your experiments.
If your protein expression is your goal, I would go for HEK293T or HEK293E (HEK293E preferred).
thanks all friends help,the reason they are both ok is that the vector both have sv40 promotor,and can express protein in the cells ,am i right ?
Just be careful that transfected plasmids may replicate in 293t because they have a SV40 promoter (typically contains a functional SV40 replication origin) and that may give you false "amplification" results that you will not get in other cell types. I dont know if it is what you like it to be.