microbiology - microbial polyphosphate (Mar/02/2002 )

I would like to have protocols to evidence and/or quantify polyphosphates inside microbial cells.
Thank you.

You could try

A novel method for determination of inorganic plyphosphates using the fluorescent dye fura-2.
Anal. Biochem. 246, 1997

which uses quenching of fura-2 emission in the presence of PPi. It works better than toluidine blue.