Biotin-streptavidin: how it works? - (Apr/25/2006 )

Hi everybody,
Would someone of you write me what is the biotin-streptavidin principle? How does this conjugate work? I am thinking about using flow cytometry for detection of my intracellular protein, unfortunately, the antibody is available only unconjugated or biotinylated…

Thank you.

Paja