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infection of the tumor human cell cultures with Newcastle disease virus - Human tumor tissue culture (Apr/23/2006 )

Hi for all of u................

Iam doing in tissue cultures.. i have 3 human tumor cells (Hela, MCF-7, HepG2)+ 2 Embryo Chicken

(fibroplast & Kidney) ..... i were cultured human tumor cells and it's Grew in good confluences..

i need to infect this human tumor cells (Hela, MCF-7, HepG2) with Newcastle disease virus ....

(1) There is any protocol for transfection of NDVirus to this cells???

(2) any information about cultivation of chicken cells

-loai abdelati-