LPS isolation by hot phenol\water method - (Feb/11/2006 )
QUOTE (Dr.Veeranagouda @ Jul 28 2008, 04:34 PM)
QUOTE (pito @ Jul 28 2008, 08:12 AM)
QUOTE (Dr.Veeranagouda @ Jul 28 2008, 03:40 PM)
Dear sir ,Charles D. Ellis,
Recently we have been involved in characterization phosphomannomutase a gene which affect LPS structure in many bacteria.
And hence we are looking for efficient protocol for LPS extract.
When I search on google, I found your information at http://www.protocol-online.org/biology-for...13636more1.html.
In fact I have your paper published in Mol Microbiology.It would be great help if you could able to send me detailed modified protocol for LPS extraction and also
Original paper on "hot phenol water method for LPS"Thanks in advance
Yours sincerely
veeranagouda
Dr. Y. Veeranagouda
Postdoctoral research fellow
Department of Microbiology
Changwon National University
Changwon-si, Kyoungnam 641-773
South Korea
Phone : 82-551-213-2758
Mobile : 008210 5458 1979
e-mail: veeresha_y@rediffmail.com
veereshagy@gmail.com
Recently we have been involved in characterization phosphomannomutase a gene which affect LPS structure in many bacteria.
And hence we are looking for efficient protocol for LPS extract.
When I search on google, I found your information at http://www.protocol-online.org/biology-for...13636more1.html.
In fact I have your paper published in Mol Microbiology.It would be great help if you could able to send me detailed modified protocol for LPS extraction and also
Original paper on "hot phenol water method for LPS"Thanks in advance
Yours sincerely
veeranagouda
Dr. Y. Veeranagouda
Postdoctoral research fellow
Department of Microbiology
Changwon National University
Changwon-si, Kyoungnam 641-773
South Korea
Phone : 82-551-213-2758
Mobile : 008210 5458 1979
e-mail: veeresha_y@rediffmail.com
veereshagy@gmail.com
Hallo Veeranagouda,
I doubt that he would read this, he has not been active since december this year and this is a very old post.
Maybe you could email him at: c.ellis@imperial.ac.uk
good luck with the research.
hi,
Thank you so much.
I will try.
-Charlie Ellis-
QUOTE (Charlie Ellis @ Oct 8 2008, 04:18 AM)
QUOTE (Dr.Veeranagouda @ Jul 28 2008, 04:34 PM)
QUOTE (pito @ Jul 28 2008, 08:12 AM)
QUOTE (Dr.Veeranagouda @ Jul 28 2008, 03:40 PM)
Dear sir ,Charles D. Ellis,
Recently we have been involved in characterization phosphomannomutase a gene which affect LPS structure in many bacteria.
And hence we are looking for efficient protocol for LPS extract.
When I search on google, I found your information at http://www.protocol-online.org/biology-for...13636more1.html.
In fact I have your paper published in Mol Microbiology.It would be great help if you could able to send me detailed modified protocol for LPS extraction and also
Original paper on "hot phenol water method for LPS"Thanks in advance
Yours sincerely
veeranagouda
Dr. Y. Veeranagouda
Postdoctoral research fellow
Department of Microbiology
Changwon National University
Changwon-si, Kyoungnam 641-773
South Korea
Phone : 82-551-213-2758
Mobile : 008210 5458 1979
e-mail: veeresha_y@rediffmail.com
veereshagy@gmail.com
Recently we have been involved in characterization phosphomannomutase a gene which affect LPS structure in many bacteria.
And hence we are looking for efficient protocol for LPS extract.
When I search on google, I found your information at http://www.protocol-online.org/biology-for...13636more1.html.
In fact I have your paper published in Mol Microbiology.It would be great help if you could able to send me detailed modified protocol for LPS extraction and also
Original paper on "hot phenol water method for LPS"Thanks in advance
Yours sincerely
veeranagouda
Dr. Y. Veeranagouda
Postdoctoral research fellow
Department of Microbiology
Changwon National University
Changwon-si, Kyoungnam 641-773
South Korea
Phone : 82-551-213-2758
Mobile : 008210 5458 1979
e-mail: veeresha_y@rediffmail.com
veereshagy@gmail.com
Hallo Veeranagouda,
I doubt that he would read this, he has not been active since december this year and this is a very old post.
Maybe you could email him at: c.ellis@imperial.ac.uk
good luck with the research.
hi,
Thank you so much.
I will try.
Hello Veeranagouda,
Yes, I haven't been active on this site for a while. I think the protocol I attached to this thread should be detailed enough for you to do your experiments, unless you have any specific technical issues? I am happy to help if you have an further queries.
Best of Luck,
Charlie.
-Charlie Ellis-