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Do transfected vectors bind histones and/or methylate? - (Feb/08/2006 )

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well yes and no. First, I'm not aware of any direct evidence that involves measurement of the binding (though I'm sure there is some). However, the theory goes (for mdr1 at least) that the plasmid associates with the histone proteins. Adding an HDAC inhibitors prevents deacetylation. This in itself does not increase promoter activity unless there is some background HAT activity.

What supposedly happens is that the transcription factor NF-Y binds to the promoter and recruits PCAF which has innate HAT activity. The action of acetylation is what then leads to promoter upregulation. Without histone association with the plasmid, there would be no effect. QED


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