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Plant nuclei isolation - (Jan/18/2006 )

I want to isolate nuclei from a cell suspension of BY2 cells. Then I want to prepare nuclear protein complexes from those nuclei.
Does anybody have a good protocol to do that
Thanks in advance


Nobody have done a similar experiment??


QUOTE (Fredo @ Jan 19 2006, 04:06 PM)
Nobody have done a similar experiment??

Rushton, P.; Weisshaar,B. (2002) DNA-protein interactions, In: Molecular Plant Biology: Practical Approach, Edts. Gilmartin, P.M.; Bowler, C., Oxford University Press, Oxford, 77-96

I did crude nuclear extracts with suspension cultures of Beta vulgaris and Nicotiana tabacum and tested topoisomerase activity. Worked well.

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