How to eliminate primer dimer in MSP - (Jan/04/2006 )
I'm trying to run multiplexe pcr of buffalo primer. When I run separate pcr primer, my gel are really nice. But when I try to run the multiplex pcr, I only obtain primer-dimer except 1 time. I change annealing temperature and increase MgCl2 but nothing work. I check primer-dimer formation with the software AUTODIMER, and normally I should not have any dimer.
Primers are not new they have been alreayd use in many study but never in mutiplex pcr.
Did you have any idea to solve the problem?