Toxicity of DMSO? - (Dec/01/2005 )
I am presently using 10% DMSO as a cyroprotectant at -80C. The sorted cells are stored for analysis (10K Array) at a later date. Unfortunately, the amount of DNA extracted downstream is very minimal after thawing.
1) What happens cellularly when the DMSO is older than six months (post-expiration)?
2) What happens cellularly if more than 10% DMSO is used?
I have read of the toxicity of DMSO to particular lines of cells...but nothing is definitive.
I am trying to clear up some past issues with archived cases and any assistance would be greatly appreciated.
Thank you Fred!