translation start and stop sites - (Nov/22/2005 )
I know that the start codon for a protein coding region is "ATG" and the stop codons are "TGA", "TAA" and "TAG".
But what are the features around these two sites that distinguish that they are the true start and stop sites?
For instance, for splicing, there is the branch site, and there are also certain nucleotides which are the same near the splice site.
Is there anything similar for start and stop sites?
Nothing that I know of... There are always multiple ORFs found in any transcript. I usually look at different ORFs and take the longest as the "true"... If you have information about the amino acid sequence that could also be used to determine the correct ORF.
for sure a ribosomal binding site is required, check this -> klick
besides that there are several effects, a stop codon can be read through for example, leading to a longer protein. the context does mater, as far as i know
Of course! Kozak sequences... my bad....
Thanks guys, thats exactly what I was looking for.
maybe all this disney stories made you get confused