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ERK 1/2 Phosphorylation - MAPK (Nov/08/2005 )

Has anybody used Elisa kit for ERK phosphorylation of hamsters primary hepatocytes?
If yes do you stimulate with PMA or insulin?

-Chantal-

QUOTE (Chantal @ Nov 8 2005, 02:32 PM)
Has anybody used Elisa kit for ERK phosphorylation of hamsters primary hepatocytes?
If yes do you stimulate with PMA or insulin?



I have used PMA in ERK hit kit (immunofluroscence based assay) but with human cells.

-Soni-