# Warburg-christian equation - (Nov/06/2005 )

what is the formula of Warburg-Christian equation??
and what does it used for???

-strawberry-

no body have an idea?

-strawberry-

QUOTE (strawberry @ Nov 6 2005, 08:45 PM)
no body have an idea?

i don't have the formular at hand, but i guess google will help on that. the idea is, that you measure absorption at 260nm (nucleic acid) and 280nm (protein) of a sample. by comparison a formular (Warburg-Christian) was developed, that will give you the conncentration of nucleic acid and protein in that sample.
this is particular useful, if you want to judge if a nucleic acid sample is free of proteins.

hope that helps

-Kersten-

QUOTE (Kersten @ Nov 6 2005, 11:54 AM)
QUOTE (strawberry @ Nov 6 2005, 08:45 PM)

no body have an idea?

i don't have the formular at hand, but i guess google will help on that. the idea is, that you measure absorption at 260nm (nucleic acid) and 280nm (protein) of a sample. by comparison a formular (Warburg-Christian) was developed, that will give you the conncentration of nucleic acid and protein in that sample.
this is particular useful, if you want to judge if a nucleic acid sample is free of proteins.

hope that helps

The formula is Protein concentration (mg/ml)= (1.55*A2 80)-(0.76*A 260)

-Hanna-

hi