where can I find protocols for nucleus staining? - (Oct/06/2005 )
I am about to stain Hela cell nucleus to see the localization of a protein of interest. But I don't know where I can find the protocols.
anybody knows? thanks
do you need just a nuclear stain, separate from or in conjunction with your POI stain?
we use 'vectashield' mounting medium with DAPI; after going through all the steps of you IF protocol, just like any other mounting medium you add one drop; all the nuclei are stained and your slide has been mounted properly
btw, we use this on primary keratinocytes so should work for HeLa, too?