plasma in immunoflourescence - (Aug/27/2009 )

Hi,

Has anyone ever used mouse plasma raised to their protein of interest as their primary antibody for staining cells for quick expression screening thru' immunofluorescence? If yes, do you dilute the plasma or use neat?

Thanks,
vaccine

vaccine on Aug 27 2009, 09:12 AM said:

I personally have never used plasma for staining but I've read papers where they have done it. The ranges of dilutions that I've seen range from 1:4 to over 1:100. I think 1:500 is the highest I've seen. I don't believe I ever read a protocol that called for using it neat.

Hope that helps.

-Carlton

Carlton H on Aug 31 2009, 05:04 PM said:

vaccine on Aug 31 2009, 04:59 PM said:

Thank you

I've used serum but not plasma. Always diluted.

bob1 on Sep 13 2009, 05:42 PM said:

I see. I will give it a go.

Thanks