Protocol Online logo
Top : New Forum Archives (2009-): : Stem Cell

Please, verify calculation - (Aug/06/2009 )

Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/Ál. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/Ál. Does that mean that I have to reduce the volume of the media to 76 Ál to get to 1000 cells/Ál? I used the formula:
C1V1=C2V2
1000 cells/Ál x 1 ml (1000 Ál)= 13000 cells/Ál X V2
V2= 77 Ál

-mntl-

mntl on Aug 6 2009, 01:49 PM said:

Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/Ál. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/Ál. Does that mean that I have to reduce the volume of the media to 76 Ál to get to 1000 cells/Ál? I used the formula:
C1V1=C2V2
1000 cells/Ál x 1 ml (1000 Ál)= 13000 cells/Ál X V2
V2= 77 Ál



Yes, 77 ul, rather than 1000, will yield 13,000 cells/ul.

-Dr Teeth-

Dr Teeth on Aug 6 2009, 11:49 AM said:

mntl on Aug 6 2009, 01:49 PM said:

Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/Ál. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/Ál. Does that mean that I have to reduce the volume of the media to 76 Ál to get to 1000 cells/Ál? I used the formula:
C1V1=C2V2
1000 cells/Ál x 1 ml (1000 Ál)= 13000 cells/Ál X V2
V2= 77 Ál



Yes, 77 ul, rather than 1000, will yield 13,000 cells/ul.


Thanks.

-mntl-