Can contaminated Matrigel be sterilised using a 0.22micron filter? - (Jul/18/2009 )
Hi,
I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.
Any tips would be very appreciated. Thanks in advance!
Cheers,
Sylvia
-Sylvia Peng-
Come on Sylvia - if it's contaminated, toss it.
-GeorgeWolff-
hi,
having worked with Matrigel myself I can not imagine at all that you even get it through a filter! And i guess you have no idea, if all the proteins pass through or if the composition changed,...I know it is expensive but throw it away!
Stardust
-stardust-
Sylvia Peng on Jul 18 2009, 08:29 PM said:
Hi,
I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.
Any tips would be very appreciated. Thanks in advance!
Cheers,
Sylvia
I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.
Any tips would be very appreciated. Thanks in advance!
Cheers,
Sylvia
I would buy new matrigel. But even theoratically, I don't think you can filter it through .22 micron pores.
-cellcounter-